General Cell Based Assays
Cayman Chemical Glycogen Assay Kit, 96 wells
Glycogen is the principal storage form of glucose in animal and human cells. It is made primarily by the liver and muscles, but it can also be made by glycogenesis within the brain and stomach. Cayman’s Glycogen Assay provides a simple, reproducible, and sensitive tool for assaying glycogen from tissue. In this assay, glycogen is hydrolyzed by amyloglucosidase to form β-D-glucose, which is then specifically oxidized to D-glucono-δ-lactone by glucose oxidase forming hydrogen peroxide in the process. Hydrogen peroxide, in the presence of horseradish peroxidase, reacts with 10-acetyl-3,7-dihydroxyphenoxazine (ADHP) in a 1:1 stoichiometry to generate the highly fluorescent product resorufin which is measure at an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.
Cayman Chemical L-LACTATE ASSAY KIT
A fluorescence-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva
Abcam Calcium Assay Kit (Colorimetric) (ab102505) 250 tests
Assay type: QuantitativePlatform: Microplate readerAssay time: 20 minSample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
Cayman Chemical OXYGEN CONSUMPTION RATE ASSAY
For the measurement of oxygen consumption rate in living cells
Cayman Chemical MitoCheck Complex IV Activity Assay Kit, 96 wells
Complex IV (cytochrome c oxidase) is the terminal electron acceptor in the mitochondrial electron transport chain. Complex IV functions by oxidizing cytochrome c and completing a four-electron reduction of oxygen (O2) to form water. During this process, two H+ are translocated from the mitochondrial matrix to the intermembrane space, contributing the mitochondrial membrane potential required for ATP synthesis. Common inhibitors of complex IV include potassium cyanide, azide, O2 limitation, nitric oxide, and carbon monoxide. Caymans MitoCheck Complex IV Activity Assay is designed to measure the direct oxidation of cytochrome c by complex IV, in an isolated bovine heart mitochondrial system that is supplied within the kit.
Eurofins Discoverx PathHunter C5AR1 Bioassay Kit, 10-Plate. (93-0557Y2-00060)
The PathHunter C5AR1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. C5a) based activation of C5AR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged C5AR1 and EA-tagged β-Arrestin-2. Activation of C5AR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Cayman Chemical Glutathione Peroxidase Assay Kit, 480 wells
GPX catalyzes the reduction of hydroperoxides, including H2O2, using reduced glutathione and thereby functions to protect the cell from oxidative damage. Cayman’s Glutathione Peroxidase Assay measures GPX activity indirectly by a coupled reaction with GR. Oxidized GSSG, produced upon reduction of an organic hydroperoxide by GPX, is recycled to its reduced state by GR and NADPH. The oxidation of NADPH to NADP+ is accompanied by a decrease in absorbance at 340 nm. The rate of decrease in the absorbance at 340 nm is directly proportional to the GPX activity in the sample. Cayman’s GPX Assay can be used to measure all of the glutathione-dependent peroxidases in plasma, erythrocyte lysates, tissue homogenates, and cell lysates.
